Stimulation of Hydrolysis of Phosphatidic Acid by Cholinergic Agents in Guinea Pig Synaptosomes
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چکیده
منابع مشابه
Stimulation of hydrolysis of phosphatidic acid by cholinergic agents in guinea pig synaptosomes.
“Light” nerve endings from guinea pig cerebral cortex were incubated with [32P]orthophosphate, myo[3H]inositol, [3H]glycerol, or [3H]glucose in the presence or absence of cholinergic agents. Acetylcholine and carbamylcholine increased incorporation of “Pi into phosphatidic acid 100% and into various classes of phosphatidylinositol 20 to 70%. These agents also stimulated the labeling of phosphat...
متن کاملSti-tes Hydrolysis of 32p-labeled Phosphatidic Acid in Guinea Pig Synaptosomes
[3H]-inositol or [3H]-arachidonate was injected intracerebrally into guinea pigs. Labeled nerve endings were incubat?$ with ACh* or CCh, both of which stimulate labeling of PhA and Phi from Pi by > 100% and 70% respectively. The 4 r addition did not affect the in vivo labeled phosphatidyl-[3H]-inositol or [ HI-arachidonyl-diglyceride and -Phi. Enhanced hydrolysis of [3H]-inositol-PhIP and -PhIP...
متن کاملCholinergic stimulation of phospholipid labelling from (32P) orthophosphate in guinea-pig cortex synaptosomes in vitro: subsynaptosomal localization.
Subsynaptosomal localization of stimulation of phospholipid labelling by cholinergic agents was investigated. Synaptosomes prepared from guinea-pig cortex were incubated with [32P]orthophosphate in the presence or absence of M carbamylcholine. Following incubation and osmotic shock, lysed synaptosomes were subjected to density gradient fractionation. Subsynaptosomal fractions were examined by e...
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The cornea has one of the highest acetylcholine (ACh) concentrations of any tissue but the function of the ACh has remained enigmatic. During studies on corneal arachidonic acid metabolism, we observed that ACh stimulates formation of labeled phosphatidic acid in rat corneas whose phospholipids were prelabeled with [14C]arachidonate. ACh did not affect the metabolism of free [14C]arachidonate. ...
متن کاملRelationship between stimulated phosphatidic acid production and inositol lipid hydrolysis in intestinal longitudinal smooth muscle from guinea pig.
Accumulation of [32P]phosphatidic acid (PA) and total [3H]inositol phosphates (IPs) was measured in the longitudinal smooth-muscle layer from guinea-pig small intestine. Stimulation with carbachol, histamine and substance P produced increases in accumulation of both [3H]IPs and [32P]PA over the same concentration range. The increase in [32P]PA accumulation in response to carbachol (1 microM-0.1...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)42918-9